FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8830296
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA270064630.583867177272428No Hit
TCCGGAAG209083423.67796051230899No Hit
AGTTCGGC195556922.14613190769596No Hit
GTAATCTT171180119.385544946624666No Hit
CAGCATCC400610.4536767510398293No Hit
AGTTCGGG323310.3661372166912638No Hit
TCCGGATG193230.21882618657404013No Hit
GCCGGAAG133420.1510934627785977No Hit
GGAATCTT123910.14032372187749992No Hit
CCGCATCA123570.13993868382215047No Hit
CAGCAGCA96560.10935080771924292ABI Solid3 Adapter A (100% over 8bp)
CAGCATCT95330.10795787593077287RNA PCR Primer, Index 43 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)