FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7635525
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT221282428.98063983812508No Hit
CCTGTGCG205859226.96071324499625No Hit
TACTCTTC171435322.452326460852397No Hit
ATGAGAAA117105015.336862887620695No Hit
GGCCACGT801041.0490961656205695TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT496700.6505119163384312No Hit
CCGGTGCG336410.44058529046791145No Hit
CCTGGGCG281490.3686583437288202No Hit
TACTCGTC208590.27318357283880285No Hit
TACTGTTC185500.24294334705210183No Hit
ATGAGAAC179920.2356354016259524No Hit
CGTGTGCG141220.18495126399298017No Hit
GGACACGG123950.16233330386581146No Hit
TACGCTTC107300.14052733767488157No Hit
CCTGTGGG105650.138366386070375No Hit
TAGTCTTC83210.10897744424908569No Hit
GGGCACGT82280.10775945334472745No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)