FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10120651
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT301010829.742236937129835No Hit
CCTGTGCG276589327.329200463487975No Hit
TACTCTTC228486622.57627498468231No Hit
ATGAGAAA158594715.670404996674622No Hit
GGCCACGT779840.7705433178162155TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT387960.38333502459476176No Hit
CCGGTGCG311940.3082212794414114No Hit
CCTGGGCG260630.2575229597384595No Hit
TACTCGTC192410.19011622868924144No Hit
TACTGTTC190930.18865387216691892No Hit
ATGAGAAC141630.139941590713878No Hit
GGACACGG132190.1306141274904154No Hit
CGTGTGCG126580.12507100580782798No Hit
TACGCTTC102620.10139663940590382No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)