FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7839031
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT225570828.77534225850108No Hit
CCTGTGCG211177226.939196949214768No Hit
TACTCTTC174841222.303930166879045No Hit
ATGAGAAA119689315.268379471901566No Hit
GGCCACGT845981.0791895069683997TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT549160.7005457689859882No Hit
CCGGTGCG372580.47528833602010245No Hit
CCTGGGCG306980.39160452356930336No Hit
TACTCGTC224920.286923217933441No Hit
TACTGTTC204160.26044035289565765No Hit
ATGAGAAC201800.25742977671602524No Hit
CGTGTGCG160140.20428545313827692No Hit
GGACACGG145000.1849718415452114No Hit
TACGCTTC119460.15239128407579966No Hit
CCTGTGGG119180.1520340970714365No Hit
TAGTCTTC93060.11871365223584393No Hit
GCTGTGCG90580.11554999591148447No Hit
GGGCACGT90100.11493767533257619No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)