FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9560946
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT281666629.460118276998948No Hit
CCTGTGCG262288527.433320928703083No Hit
TACTCTTC215909322.582420191474778No Hit
ATGAGAAA149805115.668439085421046No Hit
GGCCACGT766300.801489727062573TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT384820.4024915526141451No Hit
CCGGTGCG302310.3161925608616553No Hit
CCTGGGCG247170.2585204434791285No Hit
TACTCGTC183160.19157100144692796No Hit
TACTGTTC178650.1868538950016034No Hit
ATGAGAAC139090.1454772362483796No Hit
GGACACGG130370.13635679983968113No Hit
CGTGTGCG127420.13327133110049988No Hit
TACGCTTC99430.1039959853345056No Hit
CCTGTGGG98020.10252123586933762No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)