FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6341763
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT178675928.174483972359106No Hit
CCTGTGCG170578726.897678137767052No Hit
TACTCTTC142613722.48802107552742No Hit
ATGAGAAA96419615.203910962929394No Hit
GGCCACGT723901.1414806892026712TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT506110.7980588363204364No Hit
CCGGTGCG310690.4899110862389528No Hit
CCTGGGCG270620.4267267635198604No Hit
TACTCGTC201170.31721462943348716No Hit
ATGAGAAC169710.26760697301365566No Hit
TACTGTTC158490.2499147319128766No Hit
CGTGTGCG138640.21861428754117743No Hit
GGACACGG128020.20186815559017265No Hit
CCTGTGGG111420.17569246911308417No Hit
TACGCTTC102260.1612485360931968No Hit
TAGTCTTC80440.12684170001307207No Hit
GCTGTGCG73120.11529916838582584No Hit
GGGCACGT66780.10530194836987758No Hit
ATGAGCAA65820.10378817373023874No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)