FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8423942
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT244356929.00742906349545No Hit
CCTGTGCG227418826.996719588050343No Hit
TACTCTTC189291322.470631920305244No Hit
ATGAGAAA131435115.602564690022794No Hit
GGCCACGT778820.9245315316748381TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT457370.5429405852984268No Hit
CCGGTGCG345530.4101761384396996No Hit
CCTGGGCG270200.32075244582643136No Hit
TACTCGTC213710.25369357956168265No Hit
TACTGTTC195480.23205287975629463No Hit
ATGAGAAC163720.19435081580571187No Hit
CGTGTGCG145900.1731968240047237No Hit
GGACACGG144450.17147553959891937No Hit
CCTGTGGG116540.13834378251892046No Hit
TACGCTTC108520.12882329911578214No Hit
TAGTCTTC93030.11043523329101743No Hit
GGGCACGT92680.11001975084823708No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)