FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7613178
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT222822029.267935151391445No Hit
CCTGTGCG210266927.618807809301188No Hit
TACTCTTC174999922.986445345163347No Hit
ATGAGAAA118587815.57664880553167No Hit
GGCCACGT635000.8340800648559642TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT342720.45016680287785205No Hit
CCGGTGCG216070.28381051907626487No Hit
CCTGGGCG185470.24361705453359953No Hit
TACTCGTC141910.18640047559639353No Hit
ATGAGAAC121290.15931585994705497No Hit
TACTGTTC120620.15843580696523843No Hit
CGTGTGCG91370.12001558350533773No Hit
GGACACGG90850.11933255731049502No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)