FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8353995
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT242053628.974592395614312No Hit
CCTGTGCG227700727.25650422342843No Hit
TACTCTTC188312322.541586390702893No Hit
ATGAGAAA130241715.590349287975394No Hit
GGCCACGT763080.9134312385870473TruSeq Adapter, Index 20 (100% over 8bp)
GGACCCGT426660.5107257066828506No Hit
CCGGTGCG312530.3741084355449099No Hit
CCTGGGCG255760.3061529244391456No Hit
TACTCGTC201570.24128575609633474No Hit
TACTGTTC174520.20890603836846924No Hit
ATGAGAAC157110.18806570987892618No Hit
GGACACGG134710.161252191316849No Hit
CGTGTGCG134490.16098884425954288No Hit
CCTGTGGG105520.12631082494064216No Hit
TACGCTTC102900.12317460089454207No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)