FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002538622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12048776
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT345017028.635024835717754No Hit
CCTGTGCG332567027.601724855703186No Hit
TACTCTTC276450422.94427251365616No Hit
ATGAGAAA185375815.385446621299955No Hit
GGACCCGT1686961.4001090235223894No Hit
GGCCACGT772250.6409364735471885TruSeq Adapter, Index 20 (100% over 8bp)
CCGGTGCG293430.24353511095234903No Hit
CCTGGGCG259920.21572315727340272No Hit
TTCTCTTC173560.14404782693279383No Hit
CGTGTGCG171390.14224681411622225No Hit
CCTGTGGG156480.1298721131507466No Hit
GGACACGG149290.12390470202118455No Hit
TTGAGAAA127270.10562898671201125No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)