FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11490398
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT325316728.31204802479427No Hit
CCTGTGCG315915927.493904040573703No Hit
TACTCTTC263310622.915707532498004No Hit
ATGAGAAA176620815.371164688986404No Hit
GGACCCGT1662811.4471300297866097No Hit
GGCCACGT772300.6721264137238763TruSeq Adapter, Index 20 (100% over 8bp)
CCGGTGCG292070.2541861474250065No Hit
CCTGGGCG288990.25150564845534507No Hit
TTCTCTTC188500.16405001811077388No Hit
CGTGTGCG188410.16397169184217988No Hit
CCTGTGGG173770.1512306188175553No Hit
GGACACGG153820.13386829594588456No Hit
TTGAGAAA131400.11435635214724503No Hit
TCCTCTTC116240.10116272734852177No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)