FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10180616
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT285901428.082917575910926No Hit
CCTGTGCG278193527.32580228937031No Hit
TACTCTTC234041622.988942908759157No Hit
ATGAGAAA156422315.364718598560245No Hit
GGACCCGT1617321.588626857156777No Hit
GGCCACGT713370.7007139843011464TruSeq Adapter, Index 20 (100% over 8bp)
CCGGTGCG281960.27695770079138626No Hit
CCTGGGCG279810.27484584429861614No Hit
CGTGTGCG197770.19426132956984135No Hit
CCTGTGGG175790.17267128040189317No Hit
TTCTCTTC170580.1675537118775524No Hit
GGACACGG137350.13491325082883No Hit
TTGAGAAA135860.13344968516639857No Hit
TACTCGTC123230.12104375609491606No Hit
TCCTCTTC115070.11302852401072784No Hit
GGACTCGT108570.10664384159072497No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)