FastQCFastQC Report
Wed 8 May 2024
EGAF00002538631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10399987
Total Bases83.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT292585228.13322747422665No Hit
CCTGTGCG286534827.55145751624497No Hit
TACTCTTC239608923.03934610687494No Hit
ATGAGAAA159913215.376288451129794No Hit
GGACCCGT1560251.5002422599182097No Hit
GGCCACGT712640.6852316257703014TruSeq Adapter, Index 20 (100% over 8bp)
CCGGTGCG256790.24691377018067426No Hit
CCTGGGCG255210.24539453751240267No Hit
CGTGTGCG176590.1697982891709384No Hit
CCTGTGGG156070.1500674952766768No Hit
TTCTCTTC152590.1467213372478254No Hit
GGACACGG134230.12906746902664398No Hit
TTGAGAAA116000.11153860096171272No Hit
TCCTCTTC113950.10956744465161351No Hit
TACTCGTC112540.10821167372613062No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)