FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8065646
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGCCT253056031.37454830028494No Hit
TGTGCGGG214236626.56161701121026No Hit
ACCATAAC178491822.12988271491211No Hit
GTGTATTA106411513.193177583047905No Hit
CACCGCCT839421.04073498886512No Hit
GGTGCGGG553960.6868141745868837No Hit
ACCATACC416220.5160405006616954No Hit
TGGGCGGG411230.5098537674477656No Hit
CCCATAAC287000.3558301467731165No Hit
TGTGGGGG264500.32793405512714047No Hit
CAACGCCG204370.25338329998613873No Hit
GGGTATTA193580.240005574259024No Hit
CAACGCGT153170.18990419366285105No Hit
CCACGCCT120900.14989499911104456No Hit
ACCCTAAC109220.13541382798104454No Hit
CAACGCCA93320.11570058988455481No Hit
TGTGCGGA87440.10841041126773973No Hit
CAACGGCT86870.10770371027937502No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)