FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002538671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10091656
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA335090.33204659374041284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257610.2552702945879249No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATCA221670.21965671441832738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT166890.16537424581258023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC109430.10843611791761432Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCCGCCAGACCGCCGCCGCGCCGC103550.10260952216365679Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)