FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002538697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12544361
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC364349329.044867251508467No Hit
ACTTCATA302121824.08427180946084No Hit
CTAGACCT260562920.771317088211987No Hit
TGCCGTGG218585117.424968876453732No Hit
GAGATGCC2888052.3022695217396882Illumina PCR Primer Index 3 (100% over 8bp)
CTAGCCCT2569982.0487133621234275No Hit
TGCCGGGG641970.5117598257894523No Hit
GAGCTGAC401110.3197532341424167No Hit
CCTTCATA305220.2433125130885503No Hit
TGCGGTGG256750.20467363782021264No Hit
ACGTCATA217890.1736955752469177No Hit
GGCCGTGG205390.16373093854680998No Hit
ACTTCATT204700.16318089060096405No Hit
CTAGACCA168430.13426750075193147No Hit
TGGCGTGG144710.11535860614980709No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)