FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002538700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12749779
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC367107328.793228494391943No Hit
ACTTCATA304324923.869033337754324No Hit
CTAGACCT262597420.596231511150116No Hit
TGCCGTGG221759417.39319559970412No Hit
GAGATGCC3104182.4346931817406405Illumina PCR Primer Index 3 (100% over 8bp)
CTAGCCCT2761052.1655669482584754No Hit
TGCCGGGG685550.5376955945667764No Hit
GAGCTGAC451310.3539747630135393No Hit
CCTTCATA365830.2869304636574485No Hit
TGCGGTGG291550.22867063029092505No Hit
GGCCGTGG246310.19318766231163695No Hit
ACGTCATA242360.19008956939567345No Hit
ACTTCATT229340.1798776276828014No Hit
CTAGACCA177120.13892005500644364No Hit
TGGCGTGG165520.12982185808867744No Hit
AGTTCATA139790.10964111613228746No Hit
CTAGACCG129070.10123312725655872No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)