FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10281345
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC289410928.149128348479696No Hit
ACTTCATA245172323.846325553709168No Hit
CTAGACCT210803820.503523614857784No Hit
TGCCGTGG179065217.41651505712531No Hit
GAGATGCC2587852.51703449305514Illumina PCR Primer Index 3 (100% over 8bp)
CTAGCCCT2373282.308336117502136No Hit
TGCCGGGG614790.5979665111908996No Hit
GAGCTGAC436400.42445808403472507No Hit
CCTTCATA316270.30761539467842No Hit
TGCGGTGG249390.24256553981993603No Hit
GGCCGTGG212160.20635432426399464No Hit
ACGTCATA202450.19691003463068305No Hit
ACTTCATT196860.19147300280264887No Hit
CTAGACCA156230.151954826921964No Hit
TGGCGTGG143390.1394661885191091No Hit
CTAGACCG120150.11686214206409763No Hit
AGTTCATA117390.11417766838871762No Hit
ACTTCCTA110960.10792362283339389No Hit
ACTTCAGA107470.10452912532358365No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)