FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002538709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16692721
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC502567430.106978964064634No Hit
ACTTCATA406514924.3528242040348No Hit
CTAGACCT360200221.57827953872829No Hit
TGCCGTGG294718117.655485885135203No Hit
GAGATGCC2597251.5559176961023908Illumina PCR Primer Index 3 (100% over 8bp)
CTAGCCCT2310951.3844058137675697No Hit
TGCCGGGG557370.3339000274430993No Hit
GAGCTGAC321850.19280859004352854No Hit
TGCGGTGG261440.1566191635264257No Hit
CCTTCATA255090.15281511025074943No Hit
ACTTCATT239000.14317617840734292No Hit
ACGTCATA219510.13150043063680272No Hit
CTAGACCA213470.1278820870486004No Hit
GGCCGTGG178350.10684297664832473No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)