FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002538712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15710967
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC468804829.839334523457406No Hit
ACTTCATA381658624.292495808819407No Hit
CTAGACCT338049921.516810518410484No Hit
TGCCGTGG278849317.748703819440266No Hit
GAGATGCC2547961.6217715943264346Illumina PCR Primer Index 3 (100% over 8bp)
CTAGCCCT2267961.443552137815578No Hit
TGCCGGGG545230.3470378366907651No Hit
GAGCTGAC330990.21067449253760132No Hit
CCTTCATA282580.17986162150299215No Hit
TGCGGTGG256230.16308989764920262No Hit
ACTTCATT233000.1483040477393912No Hit
CTAGACCA206080.1311695199919903No Hit
ACGTCATA203570.12957190986398226No Hit
GGCCGTGG189950.12090280630084704No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)