FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002538715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13608354
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC395837029.0877941593818No Hit
ACTTCATA328075724.108404293421525No Hit
CTAGACCT287040421.09295510684099No Hit
TGCCGTGG238287417.51037634676464No Hit
GAGATGCC2731192.0069951149125016Illumina PCR Primer Index 3 (100% over 8bp)
CTAGCCCT2434041.7886365977839789No Hit
TGCCGGGG614270.4513918435690312No Hit
GAGCTGAC391360.28758805069297877No Hit
TGCGGTGG288790.21221523190828223No Hit
CCTTCATA287820.21150243446047917No Hit
ACTTCATT261550.1921981159514222No Hit
ACGTCATA245860.18066843352252596No Hit
GGCCGTGG210540.15471378830973972No Hit
CTAGACCA192180.14122207579256094No Hit
TGGCGTGG162310.11927232345660614No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)