FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002538719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13583622
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC396903129.21923916905226No Hit
ACTTCATA327843024.135168072256427No Hit
CTAGACCT288677921.25190910053298No Hit
TGCCGTGG239893217.660473767600422No Hit
GAGATGCC2532101.8640830847619287Illumina PCR Primer Index 3 (100% over 8bp)
CTAGCCCT2298911.6924131133802163No Hit
TGCCGGGG558490.4111495446501677No Hit
GAGCTGAC370750.27293898490402635No Hit
CCTTCATA310860.22884912433517365No Hit
TGCGGTGG256770.18902911167581077No Hit
ACTTCATT230180.16945406755282205No Hit
ACGTCATA220270.16215851707298687No Hit
GGCCGTGG205630.1513808320048953No Hit
CTAGACCA190590.14030867466718377No Hit
TGGCGTGG144430.10632657475303714No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)