FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002538842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21748302
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCAATT721171633.159903701907396No Hit
CTAGGTGA719454333.080941215548684RNA PCR Primer, Index 38 (100% over 8bp)
GATACGCC682245031.37003523309544No Hit
TCGTTCAG924110.42491133330776815No Hit
CTAGGTGN289080.13292072181083378No Hit
AGCCAATN286230.13161027467799555No Hit
GATACGCN275050.1264696434691775No Hit
AGCCAATA244940.11262488446224445No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)