FastQCFastQC Report
Sun 23 Apr 2023
EGAF00002539043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30470760
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG414620.1360714337285975Illumina Single End PCR Primer 1 (100% over 50bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC401480.13175910282513464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)