FastQCFastQC Report
Wed 19 Apr 2023
EGAF00002539053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28227224
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2237530.7926851042808886Illumina Single End PCR Primer 1 (100% over 50bp)
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1439710.5100430704769269No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT672640.23829477528502274No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC549400.19463479653542978No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT548280.19423801646240524No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC548070.19416362019871314No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA483200.1711822600763008No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG474880.16823475096240426No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG452080.16015744233297613No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG446870.15831170645756737No Hit
GCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACT415590.14723020584666774No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT402240.14250072908338418No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT389620.13802986790341126No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC386890.13706271647541396No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA361270.12798637230497764No Hit
CTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAA358730.12708653178222556No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC334540.11851679074074023No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG322270.11416992333358747No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA311960.11051742105422765No Hit
GACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCC308810.10940147709884614No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC288650.10225943578440445No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG288260.10212127129469054No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT286220.10139856473311012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)