FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002539089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21839376
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC559520.25619779612750837No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG370340.16957444205365574Illumina Single End PCR Primer 1 (100% over 50bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA285050.13052112844249764No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA259680.11890449617241812No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG220220.10083621436802956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)