FastQCFastQC Report
Wed 19 Apr 2023
EGAF00002539094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30924609
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC736820.23826331967527867No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT611440.19771955726263185TruSeq Adapter, Index 5 (97% over 38bp)
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA449490.14535026134041015No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA398510.12886500844683274No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT387670.12535970947926942No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG384520.1243411032294701No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC362780.11731110327053772No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG346770.1121339965850498No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG327740.10598032136800825No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT311980.10088405644837742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)