FastQCFastQC Report
Wed 19 Apr 2023
EGAF00002539095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30924609
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC668990.21632933176293354No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG640210.20702282767746555Illumina Single End PCR Primer 1 (100% over 50bp)
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA369430.11946149424233625No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA361930.11703624126662361No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT338330.1094047785697145No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG331270.10712180710191033No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC317190.10256879884883911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)