FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002539104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21072831
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC836120.39677630404761466No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA429980.20404472469788232No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT364000.17273426622175256No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT347700.16499918781676748TruSeq Adapter, Index 20 (97% over 38bp)
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC308660.1464729632197971No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC281330.13350365691254298No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA271890.12902395506327555No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT243830.1157082311342031No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC215900.10245419801449554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)