FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002539142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24179743
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT446730.18475382472013865TruSeq Adapter, Index 5 (97% over 36bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC361460.14948876834629715No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT295560.12223454980476839No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA277650.11482752318748798No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA263010.10877286826414988No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCC252950.10461236085098176No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC244810.10124590654251371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)