FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002539172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24335626
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACAGAAATCTCGTAT521560.21431953301714943TruSeq Adapter, Index 23 (97% over 37bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC377420.1550894971840872No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT263990.10847882031060141No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC255550.10501065392770255No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA251810.10347381242627578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)