FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002539196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25230070
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC996170.394834417819689No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA550780.2183030011410987No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT415980.16487469119189918No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC369800.14657113515737372No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTAT333280.13209634376757576TruSeq Adapter, Index 7 (97% over 35bp)
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC327620.12985298891362568No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA310130.12292078460345136No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC309330.12260370264529587No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT289780.11485501229287116No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT284640.11281776071172217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)