FastQCFastQC Report
Thu 5 Jan 2023
EGAF00002539201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23029430
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC2136450.9277042462622827No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1256280.5455106791614035No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC790960.34345617759536384No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA711930.309139218816966No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC637080.27663732884400527No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG552760.2400233093046593No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT530110.23018806805031647No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG495070.21497275442770403No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC494270.21462537283814664No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT478870.20793827723916744No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC454490.19735182329740686No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT452780.196609295149728No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC436840.18968771697779754No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC416650.1809206741113436No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC409850.17796793060010604No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA392220.17031250882023566No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG364410.15823665631324788No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG360380.15648672155585266Illumina Single End PCR Primer 1 (100% over 50bp)
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT352120.1529000066436729No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG333880.14497970640176505No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT332410.1443413927309534No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC319780.13885710588581654No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC299200.1299207144944534No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTA287650.12490539279521899No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG284880.12370258404137663No Hit
GTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCG283870.12326401478456046No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT282230.12255188252596787No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG274880.11936031417190959No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA274110.11902595939196063No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT250760.10888675924675513No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA240770.10454883164715757No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA233870.10155266543722533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)