FastQCFastQC Report
Wed 19 Apr 2023
EGAF00002539214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34003176
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC768290.2259465409937001No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT511720.15049182464602717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTAT495380.14568639117710652TruSeq Adapter, Index 9 (97% over 38bp)
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC422450.12423839467230943No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA420780.12374726407909661No Hit
CTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGC395630.11635089616334662No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT369200.10857809282285866No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA347880.10230809027956683No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT341640.10047296758396922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)