FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002539216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22991095
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTAT1393860.6062608153287176TruSeq Adapter, Index 7 (97% over 36bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1117790.48618388989302164No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA677530.2946923580629805No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC486120.2114383851660828No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA409260.17800805050825114No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG394050.1713924456403664No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT389550.16943516609365497No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC354180.15405094885650292No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC337380.1467437718821135No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA279880.1217340887852449No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT275000.11961152785458892No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG269190.11708446248427924No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC267920.11653207470109622No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT266540.11593184230677138No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC260320.11322644702220577No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG259190.11273495238047601No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA251080.10920749968629158No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT234520.10200471095439342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)