FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002539222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20302191
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC315340.1553231372909456No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTAT292730.14418640825514842TruSeq Adapter, Index 7 (97% over 35bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT225780.11120967190191443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)