FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002539246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26650058
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC770330.289053779920479No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT685190.2571063822825451TruSeq Adapter, Index 15 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA464540.17431106528923876No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT428620.16083267060807147No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA361350.13559069927727738No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA313560.11765828051856397No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT296270.11117048976028496No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC291920.10953822314382956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)