FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002539252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23488325
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC934950.39804881787015467No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA559490.23819919044887194No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC437270.18616482869681No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT420470.17901233910889772No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT413630.17610025406239058No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT412710.1757085701087668No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC411720.1752870841151934No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA396830.16894776447447826No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTAT378380.16109279823061032TruSeq Adapter, Index 1 (97% over 37bp)
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT352720.15016822187193No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG329540.14029948921432245No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA321140.1367232444203663No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG315270.1342241304988755No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA309320.1316909571031566No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC306500.13049036063661415No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA294350.12531757798821327No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG277500.1181438012289084No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG248120.10563545931861892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)