FastQCFastQC Report
Thu 5 Jan 2023
EGAF00002539262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22453657
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1170090.5211133313384096No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT440000.19595917048167255TruSeq Adapter, Index 10 (97% over 38bp)
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA439630.1957943866337675No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT419720.1869272341694718No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC380360.16939779564638402No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG334910.14915610405912944No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG305860.13621834518982812No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG286560.1276228633937002No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC268690.11966424890163772No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGCACGATGTCTAGTG253050.11269879111451644No Hit
GCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACT241130.1073900790414675No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG232060.10335064795903848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)