FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002539263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22453657
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1048410.4669217134652052No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG454500.20241691587254582Illumina Single End PCR Primer 1 (100% over 50bp)
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT389610.17351739184401008No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA362720.16154161435707334No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC359550.1601298176061031No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG266310.1186042879340323No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG258200.11499240413265421No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC258010.11490778539994621No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG257830.11482762028474916No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGCACGATGTCTAGTG230250.10254454318955705No Hit
GCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACT227940.10151575754452827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)