FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002539389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22369699
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC885770.395968671728663No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA500250.2236284001854473No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG445500.1991533279012829Illumina Single End PCR Primer 1 (100% over 50bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT392010.17524151755461706No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA336520.1504356406404932No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT303120.1355047289639436No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC282980.12650147862964092No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC277080.12386398225563965No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA260600.11649687373978523No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA239110.10689012847244837No Hit
GTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGG223880.10008181156125524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)