FastQCFastQC Report
Thu 5 Jan 2023
EGAF00002539420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22706079
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1000360.4405692413912592No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC757100.33343493608033337No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA621190.2735787187210967No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT512390.22566203526377232No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC434200.19122632313575585No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA397680.17514252460761717No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT318550.14029282642767163No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG310820.1368884517665952No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA293550.12928255908913203No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT285250.1256271503327369No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC253190.11150758349779369No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG249270.10978117357911069No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG246020.10834983882510053No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG241940.10655296319545086No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG239130.10531540914659904No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA234630.1033335610256619No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC230810.10165119217633305No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG230070.10132528826311228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)