FastQCFastQC Report
Thu 5 Jan 2023
EGAF00002539430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22166669
Sequences flagged as poor quality0
Sequence length126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG1691170.7629337542776499TruSeq Adapter, Index 6 (97% over 35bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA289840.13075487345437423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)