FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002539432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39255733
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG1252940.31917376246674595TruSeq Adapter, Index 7 (97% over 34bp)
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC555300.14145704526775743No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG521670.13289014371480468No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA467320.11904503222497462No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG449990.11463039041966175No Hit
GGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCA447260.11393495059689752No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG441790.11254152355275088No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG428730.10921462095740259No Hit
GTCCTTTAGTGTTGTGTATGGTTATCATTTGTTTTGAGGTTAGTTTGATT411760.10489168550234433No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG393720.10029617839514039No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)