FastQCFastQC Report
Thu 5 Jan 2023
EGAF00002539435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002539435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22534184
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC2154530.956116272060262Illumina Single End PCR Primer 1 (96% over 33bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN450500.19991848828428846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)