FastQCFastQC Report
Sat 13 May 2023
EGAF00002540157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32958917
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA671250.20366263855089656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT597300.18122561490718886Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC542520.16460492315326988No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG470160.14265031827350394No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT463770.1407115409769077No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA453860.13770476742303153No Hit
ATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAG449630.1364213514661298No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG447770.13585701253472618No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC373320.11326828487720031No Hit
GTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAG371480.11271001410634943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)