FastQCFastQC Report
Sat 13 May 2023
EGAF00002540158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32641721
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA661580.2026792643684443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT594840.1822330385092134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC541560.16591036973816423No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG470710.14420501909197742No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT457600.14018868674234425No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA450710.1380778911749169No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG444210.13608657460187223No Hit
ATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAG442270.1354922431939174No Hit
GTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAG367450.11257065765619405No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC365080.11184459299802237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)