FastQCFastQC Report
Sun 14 May 2023
EGAF00002540175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34145091
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA596520.17470154055234471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT518330.15180220196220887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC385650.11294449325087463No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT361240.10579558859573696No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG349880.10246860961653317No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG349430.10233681907598371No Hit
ATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAG348340.10201759309998618No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA342840.10040681982660406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)