FastQCFastQC Report
Sun 14 May 2023
EGAF00002540176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33830292
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA584920.17289830073000848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT516680.1527270293735567Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC379860.11228398501555942No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT359310.10620954734886712No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG346760.10249985427261463No Hit
ATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAG346200.10233432215128384No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG345030.101988478254932No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA340200.10056076370845396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)