FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002540224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002540224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21072247
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC3295601.563952814334418No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT2453521.1643371492370984TruSeq Adapter, Index 19 (97% over 40bp)
GTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCG2403161.1404384164631327No Hit
GCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCC2237151.0616570696043948No Hit
GTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCT1942270.9217194540287991No Hit
GGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCG1762290.836308534158697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590960.7550025395962756No Hit
GGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCC1298140.6160425131690986No Hit
GGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGG1281320.6080604503164755No Hit
CGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGC1144650.5432026304551194No Hit
CGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCC1007520.47812651398780587No Hit
GTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCT819910.389094717805842No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG781980.3710947389711216No Hit
GGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCC739690.3510256879581945No Hit
CGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCC711440.33761942900536424No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCC599360.2844309864059585No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT579280.2749018649980707No Hit
GCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGC532910.2528966180018676No Hit
ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC527740.2504431539740399No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA522560.24798494436782179No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA520710.24710701236560104No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG490720.2328750227728443No Hit
CCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCG483840.22961006484026122No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC481200.2283572321452003No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT472720.22433298167015603No Hit
CCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCC467370.22179409723130142No Hit
CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC462710.21958265770138327No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA444090.21074639073849125No Hit
CGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCC420270.1994424230126004No Hit
GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG416820.19780519846791847No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA386100.1832267816526638No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT379070.179890640044225No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG367010.17416747250542383No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG363730.17261092279337842No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC358690.1702191512846257No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC357000.16941714853665107No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG354680.16831617435008236No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG348400.16533595112092223No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT335990.1594466883384577No Hit
CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC324960.15421231537386593No Hit
GCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGC323510.15352420650726048No Hit
GCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCG323110.15333438337164518No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA320890.1522808649689803No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA319620.15167817651340174No Hit
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG312600.14834678048335329No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC307760.1460499205424082No Hit
GGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGC307390.14587433414196407No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC305830.14513402391306443No Hit
CCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGA304560.14453133545748587No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC299110.14194499523472745No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG297960.14139925371983347No Hit
GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC295350.14016065775994369No Hit
CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA291020.13810582231690813No Hit
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG285830.13564286713229964No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA285470.13547202631024588No Hit
TCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGT285160.13532491338014405No Hit
GGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCC278720.13226876089673778No Hit
CCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCG272780.12944988733285062No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCC264580.12555851305273708No Hit
GGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCC258530.12268743812655575No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA254780.12090784623016236No Hit
GCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGG250380.11881979173839412No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG246260.11686461344155656No Hit
CCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTC245600.11655140526779133No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT242080.11488096167437674No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA240410.11408845008318287No Hit
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGG237920.11290680106397766No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA237490.11270274119319122No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG236160.11207157926727035No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT233360.11074281731796329No Hit
AACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGG231480.10985064858057139No Hit
TCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGT226200.10734498319044951No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT224250.10641959540432494No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA220460.10462102119437001No Hit
CTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTG218150.10352479258619168No Hit
GGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGAC215720.10237161703732878No Hit
GACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGG214950.10200620750126933No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT213370.1012564061155889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)